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R&D Systems serum gas6

(A) Expression analysis by qPCR of genes encoding the known γ-carboxylated proteins. <t>Gas6</t> is highlighted in red. (B) Gene expression analysis by qPCR of the TAM receptors Axl , Mertk and Tyro3 in bone marrow derived monocytes (d0: day 0) and in differentiating osteoclasts cultures in the presence of RANKL and M-CSF (d2-d6: day 2 to 6). (C) Western blot analysis of the phosphorylation (P) of AKT (S473) and TAM (Y702 in AXL and Y753 in MerTK) in bone marrow derived monocytes (BMMC) serum starved for 3h and treated with GAS6 (200ng/mL) for the indicated times. Total AKT, MerTK and AXL were used as loading controls. (D) Quantification of P-AKT (S473), P-MerTK (Y753) and P-AXL (Y702) normalized to the amount of total protein (n=2-3). Results represent the mean ± SEM.

Serum Gas6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/serum gas6/product/R&D Systems
Average 93 stars, based on 13 article reviews

serum gas6 - by Bioz Stars, 2026-05

93/100 stars

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1) Product Images from "Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6"

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6

Journal: bioRxiv

doi: 10.1101/2025.09.08.673085

(A) Expression analysis by qPCR of genes encoding the known γ-carboxylated proteins. Gas6 is highlighted in red. (B) Gene expression analysis by qPCR of the TAM receptors Axl , Mertk and Tyro3 in bone marrow derived monocytes (d0: day 0) and in differentiating osteoclasts cultures in the presence of RANKL and M-CSF (d2-d6: day 2 to 6). (C) Western blot analysis of the phosphorylation (P) of AKT (S473) and TAM (Y702 in AXL and Y753 in MerTK) in bone marrow derived monocytes (BMMC) serum starved for 3h and treated with GAS6 (200ng/mL) for the indicated times. Total AKT, MerTK and AXL were used as loading controls. (D) Quantification of P-AKT (S473), P-MerTK (Y753) and P-AXL (Y702) normalized to the amount of total protein (n=2-3). Results represent the mean ± SEM.

Figure Legend Snippet: (A) Expression analysis by qPCR of genes encoding the known γ-carboxylated proteins. Gas6 is highlighted in red. (B) Gene expression analysis by qPCR of the TAM receptors Axl , Mertk and Tyro3 in bone marrow derived monocytes (d0: day 0) and in differentiating osteoclasts cultures in the presence of RANKL and M-CSF (d2-d6: day 2 to 6). (C) Western blot analysis of the phosphorylation (P) of AKT (S473) and TAM (Y702 in AXL and Y753 in MerTK) in bone marrow derived monocytes (BMMC) serum starved for 3h and treated with GAS6 (200ng/mL) for the indicated times. Total AKT, MerTK and AXL were used as loading controls. (D) Quantification of P-AKT (S473), P-MerTK (Y753) and P-AXL (Y702) normalized to the amount of total protein (n=2-3). Results represent the mean ± SEM.

Techniques Used: Expressing, Gene Expression, Derivative Assay, Western Blot, Phospho-proteomics

(A-B) Representative TRAP staining (left) and quantification of the TRAP+ osteoclast area (right) in WT osteoblasts (OB) and bone marrow cells (BM) co-cultures at day 8 in the presence of PGE 2 and VitD 3 , with or without the TAM inhibitors LDC1267 (A) or R428 (B), at the indicated concentrations. (C-F) Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant carboxylated GAS6 at the indicated concentrations for up to 6 days. (C) Representative TRAP staining at day 5 and 6 of differentiation. (D) Quantification of the TRAP+ osteoclast area at day 4, 5 and 6 of differentiation. (E) Quantification of the number of TRAP+ multinucleated osteoclasts at day 4, 5 and 6 of differentiation. (F) Quantification of the number of nuclei per osteoclast at day 5 of differentiation. Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s post tests was used in (A-B) and (D-F). ***p < 0.001, **p < 0.01, *p < 0.05, ns : non-significant.

Figure Legend Snippet: (A-B) Representative TRAP staining (left) and quantification of the TRAP+ osteoclast area (right) in WT osteoblasts (OB) and bone marrow cells (BM) co-cultures at day 8 in the presence of PGE 2 and VitD 3 , with or without the TAM inhibitors LDC1267 (A) or R428 (B), at the indicated concentrations. (C-F) Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant carboxylated GAS6 at the indicated concentrations for up to 6 days. (C) Representative TRAP staining at day 5 and 6 of differentiation. (D) Quantification of the TRAP+ osteoclast area at day 4, 5 and 6 of differentiation. (E) Quantification of the number of TRAP+ multinucleated osteoclasts at day 4, 5 and 6 of differentiation. (F) Quantification of the number of nuclei per osteoclast at day 5 of differentiation. Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s post tests was used in (A-B) and (D-F). ***p < 0.001, **p < 0.01, *p < 0.05, ns : non-significant.

Techniques Used: Staining, Derivative Assay, Cell Culture, Recombinant

(A-D) Gene expression analysis by qPCR of osteoclasts differentiation markers Acp5 (TRAP), Clcn7 , Ctsk and Dcstamp . Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant carboxylated GAS6 at the indicated concentrations for 2, 4 and 6 days (n=4 per condition). (E) Schematic representation of the assay used to assess the impact of GAS6 on pre-osteoclasts fusion in culture using a conditionally activated tdTomato (Tom) reporter. (F) Representative pictures of live osteoclasts cultures at the indicated time and concentration of recombinant GAS6. The stars indicate the presence of fusion events (Tom+ cells) in the GAS6 conditions at Day 4. (G) Quantification of the number of fusion events per 10 mm 2 at the indicated time of osteoclasts differentiation (n=16 fields per condition). Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s post tests was used in (A-D) and (G). ***p < 0.001, **p < 0.01.

Figure Legend Snippet: (A-D) Gene expression analysis by qPCR of osteoclasts differentiation markers Acp5 (TRAP), Clcn7 , Ctsk and Dcstamp . Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant carboxylated GAS6 at the indicated concentrations for 2, 4 and 6 days (n=4 per condition). (E) Schematic representation of the assay used to assess the impact of GAS6 on pre-osteoclasts fusion in culture using a conditionally activated tdTomato (Tom) reporter. (F) Representative pictures of live osteoclasts cultures at the indicated time and concentration of recombinant GAS6. The stars indicate the presence of fusion events (Tom+ cells) in the GAS6 conditions at Day 4. (G) Quantification of the number of fusion events per 10 mm 2 at the indicated time of osteoclasts differentiation (n=16 fields per condition). Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s post tests was used in (A-D) and (G). ***p < 0.001, **p < 0.01.

Techniques Used: Gene Expression, Derivative Assay, Cell Culture, Recombinant, Concentration Assay

(A-J) Six-month-old WT (non-transgenic littermates) and ApoE-Gas6 Tg male mice were analyzed. (A-B) GAS6 concentration in the serum (A) and bone marrow cavity (B) (n=4). (C) Representative pictures of sections from lumbar vertebrae stained with von Kossa and van Gieson. (D) Quantification of trabecular bone volume over tissue volume (BV/TV) from the L4 and L5 lumbar vertebrae sections (n=15-17). (E-H) μCT analysis of the distal femur trabecular bone (n=12). (E) Representative μCT images. (F) Quantification of trabecular bone volume (BV/TV). (G) Quantification of trabecular bone surface density (BS/TV). (H) Trabecular bone µCT derived data. Tb.Sp., Tb.N., and Tb.Th., trabecular spacing, number, and thickness, respectively; Conn.Dn., connectivity density. (I-J) Bone histomorphometry analysis of lumbar vertebrae in six-month-old WT and ApoE-Gas6 Tg male (n=19-20). (I) Representative pictures of TRAP staining. (J) Number of osteoclasts per bone perimeter (N.Oc/BPm) and osteoclasts surface over bone surface (Oc.S/BS). Results represent the mean ± SEM. Unpaired, 2-tailed Student’s t test was used in (A,B, D, F, G and J). ***p < 0.001, **p < 0.01, *p < 0.05, ns : non-significant.

Figure Legend Snippet: (A-J) Six-month-old WT (non-transgenic littermates) and ApoE-Gas6 Tg male mice were analyzed. (A-B) GAS6 concentration in the serum (A) and bone marrow cavity (B) (n=4). (C) Representative pictures of sections from lumbar vertebrae stained with von Kossa and van Gieson. (D) Quantification of trabecular bone volume over tissue volume (BV/TV) from the L4 and L5 lumbar vertebrae sections (n=15-17). (E-H) μCT analysis of the distal femur trabecular bone (n=12). (E) Representative μCT images. (F) Quantification of trabecular bone volume (BV/TV). (G) Quantification of trabecular bone surface density (BS/TV). (H) Trabecular bone µCT derived data. Tb.Sp., Tb.N., and Tb.Th., trabecular spacing, number, and thickness, respectively; Conn.Dn., connectivity density. (I-J) Bone histomorphometry analysis of lumbar vertebrae in six-month-old WT and ApoE-Gas6 Tg male (n=19-20). (I) Representative pictures of TRAP staining. (J) Number of osteoclasts per bone perimeter (N.Oc/BPm) and osteoclasts surface over bone surface (Oc.S/BS). Results represent the mean ± SEM. Unpaired, 2-tailed Student’s t test was used in (A,B, D, F, G and J). ***p < 0.001, **p < 0.01, *p < 0.05, ns : non-significant.

Techniques Used: Transgenic Assay, Concentration Assay, Staining, Derivative Assay

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Journal: bioRxiv

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6

doi: 10.1101/2025.09.08.673085

Figure Lengend Snippet: (A) Expression analysis by qPCR of genes encoding the known γ-carboxylated proteins. Gas6 is highlighted in red. (B) Gene expression analysis by qPCR of the TAM receptors Axl , Mertk and Tyro3 in bone marrow derived monocytes (d0: day 0) and in differentiating osteoclasts cultures in the presence of RANKL and M-CSF (d2-d6: day 2 to 6). (C) Western blot analysis of the phosphorylation (P) of AKT (S473) and TAM (Y702 in AXL and Y753 in MerTK) in bone marrow derived monocytes (BMMC) serum starved for 3h and treated with GAS6 (200ng/mL) for the indicated times. Total AKT, MerTK and AXL were used as loading controls. (D) Quantification of P-AKT (S473), P-MerTK (Y753) and P-AXL (Y702) normalized to the amount of total protein (n=2-3). Results represent the mean ± SEM.

Article Snippet: Serum GAS6 was quantified using a mouse GAS6 ELISA (DuoSet ELISA, R&D Systems, DY986) as we previously described .

Techniques: Expressing, Gene Expression, Derivative Assay, Western Blot, Phospho-proteomics

Journal: bioRxiv

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6

doi: 10.1101/2025.09.08.673085

Figure Lengend Snippet: (A-B) Representative TRAP staining (left) and quantification of the TRAP+ osteoclast area (right) in WT osteoblasts (OB) and bone marrow cells (BM) co-cultures at day 8 in the presence of PGE 2 and VitD 3 , with or without the TAM inhibitors LDC1267 (A) or R428 (B), at the indicated concentrations. (C-F) Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant carboxylated GAS6 at the indicated concentrations for up to 6 days. (C) Representative TRAP staining at day 5 and 6 of differentiation. (D) Quantification of the TRAP+ osteoclast area at day 4, 5 and 6 of differentiation. (E) Quantification of the number of TRAP+ multinucleated osteoclasts at day 4, 5 and 6 of differentiation. (F) Quantification of the number of nuclei per osteoclast at day 5 of differentiation. Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s post tests was used in (A-B) and (D-F). ***p < 0.001, **p < 0.01, *p < 0.05, ns : non-significant.

Article Snippet: Serum GAS6 was quantified using a mouse GAS6 ELISA (DuoSet ELISA, R&D Systems, DY986) as we previously described .

Techniques: Staining, Derivative Assay, Cell Culture, Recombinant

Journal: bioRxiv

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6

doi: 10.1101/2025.09.08.673085

Figure Lengend Snippet: (A-D) Gene expression analysis by qPCR of osteoclasts differentiation markers Acp5 (TRAP), Clcn7 , Ctsk and Dcstamp . Bone marrow derived monocytes (BMMC) were cultured in the presence of RANKL (20 ng/mL) and M-CSF (10 ng/mL) with or without recombinant carboxylated GAS6 at the indicated concentrations for 2, 4 and 6 days (n=4 per condition). (E) Schematic representation of the assay used to assess the impact of GAS6 on pre-osteoclasts fusion in culture using a conditionally activated tdTomato (Tom) reporter. (F) Representative pictures of live osteoclasts cultures at the indicated time and concentration of recombinant GAS6. The stars indicate the presence of fusion events (Tom+ cells) in the GAS6 conditions at Day 4. (G) Quantification of the number of fusion events per 10 mm 2 at the indicated time of osteoclasts differentiation (n=16 fields per condition). Results represent the mean ± SEM. One-way ANOVA with Bonferroni’s post tests was used in (A-D) and (G). ***p < 0.001, **p < 0.01.

Article Snippet: Serum GAS6 was quantified using a mouse GAS6 ELISA (DuoSet ELISA, R&D Systems, DY986) as we previously described .

Techniques: Gene Expression, Derivative Assay, Cell Culture, Recombinant, Concentration Assay

Journal: bioRxiv

Article Title: Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6

doi: 10.1101/2025.09.08.673085

Figure Lengend Snippet: (A-J) Six-month-old WT (non-transgenic littermates) and ApoE-Gas6 Tg male mice were analyzed. (A-B) GAS6 concentration in the serum (A) and bone marrow cavity (B) (n=4). (C) Representative pictures of sections from lumbar vertebrae stained with von Kossa and van Gieson. (D) Quantification of trabecular bone volume over tissue volume (BV/TV) from the L4 and L5 lumbar vertebrae sections (n=15-17). (E-H) μCT analysis of the distal femur trabecular bone (n=12). (E) Representative μCT images. (F) Quantification of trabecular bone volume (BV/TV). (G) Quantification of trabecular bone surface density (BS/TV). (H) Trabecular bone µCT derived data. Tb.Sp., Tb.N., and Tb.Th., trabecular spacing, number, and thickness, respectively; Conn.Dn., connectivity density. (I-J) Bone histomorphometry analysis of lumbar vertebrae in six-month-old WT and ApoE-Gas6 Tg male (n=19-20). (I) Representative pictures of TRAP staining. (J) Number of osteoclasts per bone perimeter (N.Oc/BPm) and osteoclasts surface over bone surface (Oc.S/BS). Results represent the mean ± SEM. Unpaired, 2-tailed Student’s t test was used in (A,B, D, F, G and J). ***p < 0.001, **p < 0.01, *p < 0.05, ns : non-significant.

Article Snippet: Serum GAS6 was quantified using a mouse GAS6 ELISA (DuoSet ELISA, R&D Systems, DY986) as we previously described .

Techniques: Transgenic Assay, Concentration Assay, Staining, Derivative Assay

Vitamin K metabolites and dependent proteins at baseline and at 1 and 6 weeks post-phytomenadione administration in pseudoxanthoma elasticum (PXE) patients, in non-carriers and in ABCC6 carriers.

Journal: Frontiers in Medicine

Article Title: The Effects of Parenteral K1 Administration in Pseudoxanthoma Elasticum Patients Versus Controls. A Pilot Study

doi: 10.3389/fmed.2018.00086

Figure Lengend Snippet: Vitamin K metabolites and dependent proteins at baseline and at 1 and 6 weeks post-phytomenadione administration in pseudoxanthoma elasticum (PXE) patients, in non-carriers and in ABCC6 carriers.

Article Snippet: Commercial ELISA kits were used to measure des-γ-carboxy prothrombin (DCP) (Prothrombin Induced by Vitamin K Absence or Antagonist II—PIVKA-II) in citrated plasma (Asserachrom, Diagnostica Stago, Asnière-sur-Seine, France), Fetuin A in EDTA-plasma (Quantikine, R&D System Inc., Abingdon, UK), Gas6 in serum (USCN, Life Science Inc., Houston, TX, USA), under-carboxylated (Glu) OC (EIA, Glu-Oc) in serum (Takara Bio Inc., Shiga, Japan), carboxylated (Gla) OC (EIA, Gla-OC) in heparinized plasma (Takara Bio Inc., Shiga, Japan).

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